CELL MICROSCOPY CORE

Equipment

Equipment for (immuno)-electron microscopy:

  • 1 FEI Tecnai 20 electron microscope
  • 1 Fei Tecnai 12 electron microscope
  • 1 Jeol 1010 electron microscope
  • 3 Leica ultra microtomes
  • 5 Leica ultra-cryomicrotomes
  • 1 Leica EM-pact 1 high-pressure freezer
  • 1 Balzers critical point dryer
  • 1 Leica AFS 1 freeze substitution machine
  • 1 Leica AC20 automated grid stainer.

 
Technical details of the electron microscopes:

Microscope Max acc
voltage
Filament STEM Cryostage CCD camera Plate camera Special features
Jeol 1011 100 KV tungsten - + 2048 x 2048 + -
FEI Tecnai 12 120 KV LaB6 + + 2048 x 2048 + Tomography
low dose software
FEI Tecnai 20 200 KV LaB6 - + 4096 x 4096 +

 

Equipment for live-cell imaging:

  • 1 Zeiss LSM 510 live-cell imaging station with 32 PMT meta-detector
  • 1 Zeiss LSM700
  • 1 Zeiss Live Cell Imager with Ultraview spinning disk and a Cairn monochromator
  • 1 Deltavision RT Core system equipped with lasers for FRAP, FLIP and FRET

 

Technical details light microscopes:

Optics

LSM 700

LSM 510

Ultraview

Widefield

Deltavision

Lens 100x

 

yes, DIC

yes, DIC

yes, DIC

yes, DIC

Lens 63x

yes, DIC

yes, DIC

yes, DIC

yes, DIC

yes, DIC

Lens 40x

yes

yes, DIC

yes, DIC

yes, DIC

yes, DIC

Lens 20x

          yes  

yes

yes

yes, DIC

Lens 16x

 

yes

yes

yes

 

Lens 10x

 

yes

yes

yes

 

 

Light sources

LSM 700

LSM 510

Ultraview

Widefield

Deltavision

Laserlines

405, 488, 555, 639

458, 477, 488, 514, 543, 633

488, 568, 647

 

410, 488, 532

Krypton lamp

 

 

 

monochromator

 

Mercury lamp

yes

yes

yes

yes

 

Halogen lamp

yes

yes

yes

yes

 

Xenen lamp

       

yes

LED lamp

       

yes

 

Detectors

LSM 700

LSM 510

Ultraview

Widefield

Deltavision

Detector

2 PMT

2 PMT + metadetector

Orca ER

Cascade 650 CCD

Coolsnap HQ + Photometrics EMCCD

Max resolution

2048 x 2048

2048 x 2048

1344 x 1024

653 x 492

1392 x 1024 + 1024 x 1024

Frames/sec

5

4

12 - 45

27 - 555

max 30 +

 

Software / techniques

LSM 700

LSM 510

Ultraview

Widefield

Deltavision

Software

Zen2010

Zen2008

Ultraview

Metamorph

Softworx

 

   

(Metamorph)

   

FRAP

no

yes

no

no

yes

FLIP

no

yes

no

no

no

FRET

no

yes

yes

yes

yes

time series

yes

yes

yes

yes

yes

3D

yes

yes

yes

yes

yes

Deconvolution

no

no

no

yes

yes

Panels

yes

yes

no

yes

yes

Point visiting

yes

no

no

yes

yes

 

Additional features

LSM 700

LSM 510

Ultraview

Widefield

Deltavision

Temp control

no

RT- 40°C

RT- 40°C

RT- 40°C

RT- 40°C

CO2 control

no

yes

yes

yes

yes

Autom. Z scan

z stepper

z stepper

Pifoc

Pifoc

DV Stage

Motorized stage

yes

yes

yes

yes

yes

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