Cryosectioning and Immuno-Electron Microscopy
Date: 13 June - 16 June 2022 (17 June 2022 optional CLEM day)
We are taking all measures with respect to the Coronavirus pandemic to make the EM workshop a safe experience.
Currently, most corona restrictions have been lifted in the Netherlands.
For more information on the current situation and regulations you can check:
https://www.government.nl/topics/coronavirus-covid-19/tackling-new-coronavirus-in-the-netherlands/coronavirus-measures-in-brief and https://www.rivm.nl/en/coronavirus-covid-19
The following conditions may apply, depending on government advice and regulations, and the organizer's judgements on the current situations. Please be sure you are willing to comply before registering:
- Registration is limited to participants who are currently permitted to enter Netherlands. You can find out more about travel regulations to the Netherlands here: https://reizentijdenscorona.rijksoverheid.nl/en
- You may be required to take a fast-COVID and have a negative result each morning before entering to the lab. We will supply tests.
The 4 day workshop is organized in collaboration with Leica Microsystems and is meant for people that do not have experience with the Tokuyasu technique. Experience in resin sectioning is not necessary, but is definitely an advantage. The workshop is primarily practical in nature, although some topics will be dealt with in lectures. The entire procedure from (live) specimen till immuno-labeled ultrathin section will be covered from start to finish. Most of the time will be spent in the lab working with standard specimens like cultured cells or tissues. Besides managing the ultra cryo-microtome, the preparation of tools and other necessities will be covered.
We provide an excellent learning environment. When manual skills like ultrathin sectioning are being trained the participants will have their own ultra-cryo microtome (often equipped with a camera) to practice on. The number of participants is limited to 12 people. We will provide you with a practical manual covering the topics of the course.
A preliminairy prgram and a more elaborate list of topics that are addressed in this course can be found here.
Available equipment: 2 x Reichert Ultracut E, 3x Leica Ultracut S, 2x Leica Ultracut T, 1x Leica UC6/FC6 ,1 x Leica UC6, 2x Leica UC7. Leica KMR3, Leica AC20, Leica EM AMW, Leica EM TRIM2, Leica EM ACE600, Edwards Auto 306 carbon evaporator, JEOL electron microscopes, 2 FEI electron microscopes.
Teachers & lecturers:
Cell Microscopy Core: Suzanne van Dijk, Despina Xanthakis, Corlinda ten Brink, Nalan Liv, Tineke Veenendaal, Cilia de Heus
Leica Microsystems: Jan de Weert
Price: € 1400,- excluding hotel and dinners, including lunches. (+ € 350,- for optional extra day)
For registration and further information: https://webwinkel.umcutrecht.nl/en/product/cryo-sectioning-and-immuno-electron-microscopy-workshop.
Participants are accepted on a “first-come, first-served” basis therefore we recommend that you contact us at your earliest convenience.
After the registration deadline on May 20th 2022 you will receive an invoice and additional information about hotels etc. Cancellation is possible till May 20th 2022, thereafter the course fee has to be paid in full. The CMC reserves the right to cancel this course if less than 8 participants have registered on May 20th 2022.