CELL MICROSCOPY CORE
Equipment
Equipment for (immuno)-electron microscopy:
- 1 FEI Tecnai 20 electron microscope
- 1 Fei Tecnai 12 electron microscope
- 1 Jeol 1010 electron microscope
- 3 Leica ultra microtomes
- 5 Leica ultra-cryomicrotomes
- 1 Leica EM ICE high-pressure freezer
- 1 Balzers critical point dryer
- 1 Leica AFS 2 freeze substitution machine
- 1 Leica AC20 automated grid stainer.
Technical details of the electron microscopes:
| Microscope | Max acc voltage |
Filament | STEM | Cryostage | CCD camera | Plate camera | Special features |
| Jeol 1011 | 100 KV | tungsten | - | + | 2048 x 2048 | + | - |
| FEI Tecnai 12 | 120 KV | LaB6 | + | + | 2048 x 2048 | + | Tomography low dose software |
| FEI Tecnai 20 | 200 KV | LaB6 | - | + | 4096 x 4096 | + |
Equipment for Light Microscopy:
- 1 Leica Thunder with Tirf and infinity scanner
- 1 Zeiss LSM700
- 1 ECHO Revolve, color camera and fluorescent microscope
- 1 Deltavision RT Core system equipped with lasers for FRAP, FLIP and FRET
Technical details light microscopes:
|
Optics |
LSM 700 |
Deltavision |
Thunder |
CellInsight |
Revolve |
|
Lens 100x |
|
yes, DIC |
yes, Tirf |
|
|
|
Lens 63x |
yes, DIC |
yes, DIC |
yes, Water |
|
yes |
|
Lens 40x |
yes |
yes, DIC |
yes, 2x Water, Oil |
|
yes |
|
Lens 20x |
yes |
yes, DIC |
yes |
yes |
yes |
|
Lens 10x |
|
|
yes |
yes |
yes |
|
Lens 4x |
|
|
|
|
yes |
|
Light sources |
LSM 700 |
Deltavision |
Thunder |
CellInsight |
Revolve |
|
Laserlines |
405, 488, 555, 639 |
410, 488, 532 |
488, 561 |
|
|
|
Krypton lamp |
|
|
|
|
|
|
Mercury lamp |
yes |
|
|
|
|
|
Halogen lamp |
yes |
|
yes |
yes |
yes |
|
Xenen lamp |
yes |
||||
|
LED lamp |
yes |
405, 488, 568, 633 |
405, 488, 555, 639 |
405, 488, 555, 639 |
|
Detectors |
LSM 700 |
Deltavision |
Thunder |
CellInsight |
Revolve |
|
Detector |
2 PMT |
Coolsnap HQ + Photometrics EMCCD |
Prime 95B sCMOS |
CMOS |
CMOS + Color camera |
|
Max resolution |
2048 x 2048 |
1392 x 1024 + 1024 x 1024 |
1608x1608 |
||
|
Frames/sec |
5 |
max 30 + |
60 |
|
Software / techniques |
LSM 700 |
Deltavision |
Thunder |
CellInsight |
Revolve |
|
Software |
Zen2010 |
Softworx |
LasX |
Cellomics |
Echo |
|
|
(Metamorph) |
||||
|
FRAP |
no |
yes |
yes |
no |
no |
|
FLIP |
no |
no |
yes |
no |
no |
|
FRET |
no |
yes |
yes |
no |
no |
|
time series |
yes |
yes |
yes |
no |
no |
|
3D |
yes |
yes |
yes |
yes |
yes |
|
Deconvolution |
no |
yes |
yes |
no |
no |
|
Computaional clearing |
no |
no |
yes |
no |
no |
|
Panels |
yes |
yes |
yes |
yes |
yes |
|
Point visiting |
yes |
yes |
yes |
yes |
no |
|
Additional features |
LSM 700 |
Deltavision |
Thunder |
CellInsight |
Revolve |
|
Temp control |
no |
RT- 40°C |
RT- 40°C |
no |
no |
|
CO2 control |
no |
yes |
yes |
no |
no |
|
Autom. Z scan |
z stepper |
DV Stage |
yes |
yes |
no |
|
Motorized stage |
yes |
yes |
yes |
yes |
no |